منابع مشابه
Computational detection and suppression of sequence-specific off-target phenotypes from whole genome RNAi screens
A challenge for large-scale siRNA loss-of-function studies is the biological pleiotropy resulting from multiple modes of action of siRNA reagents. A major confounding feature of these reagents is the microRNA-like translational quelling resulting from short regions of oligonucleotide complementarity to many different messenger RNAs. We developed a computational approach, deconvolution analysis ...
متن کاملParallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets
Cytokinesis involves temporally and spatially coordinated action of the cell cycle and cytoskeletal and membrane systems to achieve separation of daughter cells. To dissect cytokinesis mechanisms it would be useful to have a complete catalog of the proteins involved, and small molecule tools for specifically inhibiting them with tight temporal control. Finding active small molecules by cell-bas...
متن کاملA direct look at RNAi screens
Dynamical Cell Systems Laboratory, Division of Cancer Biology, Chester Beatty Laboratories, Institute of Cancer Research, London, UK * Corresponding author. Dynamical Cell Systems Laboratory, Division of Cancer Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. Tel.: þ 44 20 7153 5080; Fax: þ 44 20 7153 5177; E-mail: E-mail: chris.bakal@icr....
متن کاملPooled RNAi Screens - Technical and Biological Aspects
RNA interference (RNAi) screens have recently emerged as an exciting new tool for studying gene function in mammalian cells. In order to facilitate those studies, short hairpin RNA (shRNA) expression libraries covering the entire human transcriptome have become commercially available. To make use of the full potential of such large-scale shRNA libraries, microarray-based methods have been devel...
متن کاملFUN-L: gene prioritization for RNAi screens
MOTIVATION Most biological processes remain only partially characterized with many components still to be identified. Given that a whole genome can usually not be tested in a functional assay, identifying the genes most likely to be of interest is of critical importance to avoid wasting resources. RESULTS Given a set of known functionally related genes and using a state-of-the-art approach to...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2014
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.2958